Recombinant Human RANKL/TNFSF11/CD254 Protein,His-Flag Tag

Description :
Synonyms RANKL; OPGL;ODF; CD254 Source Human RANKL/TNFSF11/CD254 Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus. It contains Gly63-Asp244.[Accession | O14788-2] Molecular Weight The protein has a predicted MW of 22.6 kDa. Due to glycosylation, the protein migrates to 35-60 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 90% as determined by SDS-PAGE Activity ELISA Data: Immobilized Human RANKL, His Tag at 2g/ml (100l/well) on the plate. Dose response curve for Human TNFRSF11A, hFc Tag with the EC50 of 8.5ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.