Faecalis shown in this study and present inside the accessible literature migh ously demonstrated and suggest applicability of your oil and TQ against a broad spectrum indicate pathogens. The FAUC 365 Technical Information results of susceptibility extracts and TQ to the tested substance of bacterialon strain-dependencyconcerning NS seed these bacteria efficiency against [16,19,28,32]. shown within this study and present inside the readily available literature may well indicate E. coli, E. faecalisThe antibacterial effects of NS seed oil have been observed to be strongly rely ent around the final TQ concentration these samples. on strain-dependency of susceptibilityin the bacteria towards the tested substances [16,19,28,32].The antibacterial effects of NS seed oil have been observed to become strongly dependent around the final TQ Materials and Solutions four. concentration inside the samples.4.1. Optimization of Supercritical Carbon Dioxide 3-Chloro-5-hydroxybenzoic acid Purity extraction from Nigella sativa Seeds 4. Supplies and Solutions 4.1. Optimization of Supercritical Carbon Dioxide Extraction from Nigella sativa Seeds four.1.1. Plant Material and Extraction Equipment four.1.1. Plant Material and Extraction Gear from FZL company from Poland, batch num Nigella sativa L. seeds had been bought ber Nigella sativa L. seeds have been bought from FZL corporation from Poland, batch Gdynia, Poland F4568. The Voucher specimen No WLDK/2013 is stored at Biovico, number F4568. The had been ground with a Bosch KM13 is stored (Bosch, Munich, Germany), 10 g a The seeds Voucher specimen No WLDK/2013 grinder at Biovico, Gdynia, Poland. The seeds have been ground having a Bosch KM13 grinder (Bosch, Munich, Germany), 10 g at once for 2 min. Ground seeds (4.0 g) were put in 10 mL stainless steel extraction vessel after for 2 min. Ground seeds (four.0 g) have been place in 10 mL stainless steel extraction vessels. Supercritical CO2 extraction of NS seed oil was performed with a Waters MV-10 ASF Supercritical CO2 extraction of NS seed oil was performed with a Waters MV-10 ASFE (Waters Corporation, Milford, MA, USA) extractor at different circumstances of temperature (Waters Corporation, Milford, MA, USA) extractor at distinctive situations of temperature, pressure, extraction time, scCO2 flow (Figure 4). CO2 of 99.995 purity purity (AirLiquide pressure, extraction time, andand scCO2 flow (Figure four). CO2 of 99.995 (AirLiquide, Cracow, Poland) was used for extraction oil. Cracow, Poland) was utilized for extraction of NSof NS oil.Figure 4.four. Flow diagramaof a supercritical carbon dioxide extraction(1. CO2 supply; 2.supply; two. coolin Figure Flow diagram of supercritical carbon dioxide extraction method program (1. CO2 cooling heat exchanger; three. 3. CO2 pump; co-solvent vessel; five. co-solvent pump; 6. extraction oven with with ex heat exchanger; CO2 pump; four. 4. co-solvent vessel; five. co-solvent pump; 6. extraction oven extraction vessels;7. backpressure regulator; 8. sample collection; 9. wet 9. wet gas meter 2and CO2 release) traction vessels; 7. backpressure regulator; 8. sample collection; gas meter and CO release).four.1.2. Modelling of Oil Extraction in the Nigella sativa SeedsThe analysis with the effects of stress, temperature, extraction time and scCO2 flow on theTheextraction on the effects of stress, temperature, extraction time and scCO2 flow oil analysis efficiency from NS seeds was performed making use of a central composite around the style (CCRD) with response NS seeds was performed The experimental rotatableoil extraction efficiency from surface methodology (RSM).working with a central composit design used within this (CCRD) wi.
