ion have been had been drastically changed (log2 2). Amongst them, the expression levels of GA synthesis connected genes were drastically decreased, including GA20ox (Caspase 9 Inhibitor review Traes1A01G263600 and Traes3D01G401400), which encoded the important enzymes in the GA synthetic pathway, and terpene synthetase gene (TraesCS7A01G002400) was decreased about 2.1, 4.2, and 14.5 instances asInt. J. Mol. Sci. 2021, 22, x FOR PEER REVIEW6 ofInt. J. Mol. Sci. 2021, 22,considerably changed (log2 two). Among them, the expression levels of GA synthesis 6 of 12 associated genes were substantially reduced, which include GA20ox (Traes1A01G263600 and Traes3D01G401400), which encoded the crucial enzymes in the GA synthetic pathway, and terpene synthetase gene (TraesCS7A01G002400) was decreased about 2.1, four.2, and 14.5 occasions as compared with WT (Figure 4A), respectively. Alternatively, the of GA2ox compared with WT (Figure 4A), respectively. However, the expressionexpression of GA2ox (TraesCS3B01G166100) and gene (TraesCS6D01G166900, TraesCS6B01G074100 (TraesCS3B01G166100) and cytochrome P450cytochrome P450 gene (TraesCS6D01G166900, and TraesCS5D01G323800) TraesCS5D01G323800) which associated to GA inactivation,DELLA TraesCS6B01G074100 and which related to GA inactivation, plus the expression of as well as the (TraesCS1A01G413800 and TraesCS3B01G262400), the vital negative the crucial expression of DELLA (TraesCS1A01G413800 and TraesCS3B01G262400), regulators of GA signal transduction pathway,transduction pathway, were considerably increased with negative regulators of GA signal have been considerably elevated with 1.4, 1.six, 1.five, 1.3, 1.4 and 1.six, 1.5, 1.three, 1.4 and 1.4 occasions of WT,than that of WT, respectively. Additionally, it was 1.four, 1.4 occasions LIMK2 Inhibitor web higher than that larger respectively. Furthermore, it was discovered that the expression levelsexpression levels of GA signal transduction genes, and located that the of GA signal transduction genes, TPR (TraesCS6B01G005400 TPR TraesCS4A01G244800) wereTraesCS4A01G244800)times decreased and 2.two occasions (p(p 0.01) (TraesCS6B01G005400 and decreased about three.three have been (p 0.01) about three.3 occasions 0.01) compared with(p 0.01) outcomes indicated thatThe results indicated that TaWUS-like and and 2.2 occasions WT. The compared with WT. TaWUS-like may well inhibit the synthesis may well functiontheGA, which and function of GA, which was constant with the phenotype of inhibit of synthesis was consistent with the phenotype of dwarfing plants using a reduced GA content material in internodes. dwarfing plants using a reduced GA content material in internodes.Figure four. Expression levels the downstream GA-, BR- (A) and cell division-related genes (B) regulated by by TaWUS-likeFigure 4. Expression levels ofof the downstream GA-, BR- (A) and cell division-related genes (B) regulatedTaWUS-like-OE. OE. Red and green circles indicate the expression in the genes was changed upward and downward, respectively. Red and green circles indicate the expression with the genes was changed upward and downward, respectively.Furthermore, the expression levels of genes involved in the BR pathway in which 5 Additionally, the expression levels of genes involved in the BR pathway in which 5 genesrelated to synthesis and three connected to signal transduction had been also changed siggenes associated to synthesis and three associated to signal transduction have been also changed substantially (log2 The The two inside the inside the BR signal had been drastically changed, nificantly (log2 2). 2). two genes genes BR signal pathway pathway were significantly changed, se
