outcomes, obtained by solution ion scan mode analysis, had been observed from the product ion spectra obtained after the isolation of m/z 227.0 on Q1. This precursor ion may be the product ion spectra obtained immediately after the isolation of m/z 227.0 on Q1. This precursor ion is most likely represent the molecular ion ofionallegedalleged metabolite of five, by de-nitration of probably to to represent the molecular an of an metabolite of five, obtained obtained by denitration of your side chain. Figure 9a reports the DP Agonist manufacturer comparison from the m/z 227.0 chromatographic profiles obtained from the rat liver microsomal fraction just before the incubation with compound five (dotted line) and just after two hours’ incubation (continuous line). A chromatographic peak is evident at the retention time of two.60 min only in theAntioxidants 2022, 11,12 ofthe side chain. Figure 9A reports the comparison on the m/z 227.0 chromatographic profiles obtained in the rat liver microsomal fraction prior to the incubation with compound five (dotted line) and just after two hours’ incubation (continuous line). A chromatographic peak is evident at the retention time of two.60 min only within the second profile, viz. right after two hours’ incubation. The Bax Inhibitor Species corresponding item ion spectrum, depicted in Figure 9B, exhibits the Antioxidants 2022, 10, x FOR PEER Assessment 13 of 21 loss of consecutive fragments from the side chain and it is compatible with the supposed metabolite’s structure.Figure 9. (a) Superimposed mass chromatograms of thethe m/z 227.0 precursor ion, obtained from Figure 9. (A) Superimposed mass chromatograms of m/z 227.0 precursor ion, obtained in the rat liverliver microsomal fractiont at t0=(dotted line) and t = = 2 h (continuous line)incubation with the rat microsomal fraction at = 0 (dotted line) and t 2 h (continuous line) incubation with compound 5. (b) Item ion spectrum from the chosen m/z 227.0 precursor, collected at 2.60 min, compound five. (B) Item ion spectrum on the selected m/z 227.0 precursor, collected at two.60 min, in the latter analysis. in the latter evaluation.Analogue experiments had been executed on the rat liver microsomal fraction incubated Analogue experiments were executed around the rat liver microsomal fraction incubated with compound 7. The m/z 288.0 precursor ion isolated on Q1 corresponds towards the molecular with compound 7. The m/z 288.0 precursor ion isolated on Q1 corresponds for the molecularalleged metabolite 7 metabolite 7 obtained after single the side chain.in the side ion of the ion in the alleged obtained after single de-nitration of de-nitration Figure 10A chain. Figure 10a reports the m/z 288.0 chromatographic profiles obtained from the rat reports the comparison with the comparison in the m/z 288.0 chromatographic profiles obtained from the fraction prior to the incubationbefore compound 7 and immediately after two hours, liver microsomal rat liver microsomal fraction with all the incubation with compound 7 and after two This time, a chromatographic peak is evident at the retention time of three.78 respectively. hours, respectively. This time, a chromatographic peak is evident at the retention time of three.78 min only rat the profile from the rat liver microsomal fraction min only in the profile in the in liver microsomal fraction collected just after two hours’ collected after two hours’ incubation. The ion spectrum, depicted ion Figure 10B, exhibits incubation. The corresponding product corresponding item in spectrum, depicted in Figure 10b, exhibits a fragmentation comparable to Figure 9b. The solution ion spe
