Ch a usually accepted nighttime behavior could influence wellness and well-being.
Ch a frequently accepted nighttime behavior may possibly influence overall health and well-being.NIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptSupplementary MaterialRefer to Internet version on PubMed Central for supplementary material.AcknowledgmentsThis work was supported by the National Institute of Health Grants NS-050595, AG-020269, and AG-12914 and by the Division of Veterans Affairs Sierra-Pacific Mental Illness Study, Education and Clinical Center.
Redox Biology two (2014) 206Contents lists readily available at ScienceDirectRedox Biologyjournal homepage: elsevierlocateredoxMini BRD9 Storage & Stability ReviewA assessment with the mitochondrial and glycolytic metabolism in human platelets and leukocytes: Implications for their use as bioenergetic biomarkersPhilip A. Kramer 1, Saranya Ravi 1, Balu Chacko, Michelle S. Johnson, Victor M. Darley-Usmar nDepartment of Pathology, UAB Mitochondrial Medicine Laboratory, Center totally free Radical Biology, University of Alabama at Birmingham, Birmingham, AL, USAart ic l e i nf oArticle history: Received 30 December 2013 Accepted 30 December 2013 Offered on the web 10 January 2014 Key phrases: Reserve capacity Oxidative strain Metabolic shift Biomarker IDO2 custom synthesis leukocytes Plateletsa b s t r a c tThe assessment of metabolic function in cells isolated from human blood for treatment and diagnosis of disease is usually a new and essential location of translational study. It truly is now becoming clear that a broad array of pathologies which present clinically with symptoms predominantly in a single organ, for example the brain or kidney, also modulate mitochondrial energetics in platelets and leukocytes permitting these cells to serve as “the canary inside the coal mine” for bioenergetic dysfunction. This opens up the possibility that circulating platelets and leukocytes can sense metabolic pressure in patients and serve as biomarkers of mitochondrial dysfunction in human pathologies which include diabetes, neurodegeneration and cardiovascular disease. In this overview we will describe how the utilization of glycolysis and oxidative phosphorylation differs in platelets and leukocytes and talk about how they could be applied in patient populations. Since it is clear that the metabolic programs amongst leukocytes and platelets are fundamentally distinct the measurement of mitochondrial function in distinct cell populations is required for translational investigation. 2014 The Authors. Published by Elsevier B.V. All rights reserved.Contents Introduction. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Biological functions and metabolic programs of platelets and leukocytes . . . . Leukocytes and platelets as systemic biomarkers of metabolic strain . . . . . . . New approaches to measuring cellular bioenergetics in leukocytes and platelets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Cellular mitochondrial physiology and glycolysis in platelets and leukocytes . Differential glycolytic and oxidative metabolism in leukocytes and platelets . Future outlook. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Disclosures. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Acknowledgments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
