Recombinant Human LIGHT/TNFSF14 Trimer Protein,His-Flag Tag

Description :
Synonyms HVEM-L; TNFSF14;CD258; LTg Source Human LIGHT/TNFSF14 Trimer Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus. It contains Ser89-Val240 Trimer.[Accession | O43557-1] Molecular Weight The protein has a predicted MW of 52.4 kDa. Due to glycosylation, the protein migrates to 53-140 kDa under Non reducing (N) condition based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 90% as determined by SDS-PAGE Activity ELISA Data: Immobilized Human LIGHT Trimer, His tag at 1g/ml (100l/well) on the plate. Dose response curve for Human HVEM, hFc Tag with the EC50 of 37.5ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.