Recombinant Mouse G-CSF R/CD114Protein,His Tag

Description :
Synonyms CD114; G-CSF R;G-CSF-R; CSF3R Source Mouse G-CSF R/CD114Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Cys26-Asp626.[Accession | P40223] Molecular Weight The protein has a predicted MW of 68.47 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Mouse G-CSF R, His Tag at 0.2g/ml (100l/well) on the plate. Dose response curve for Human G-CSF, hFc Tag with the EC50 of 1.2ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.