Recombinant Human Her2/ErbB2 Domain 4 Protein,His-Avi Tag

Description :
Synonyms ErbB2; EGFR2;CD340; HER-2 Source Human Her2/ErbB2 Domain 4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Pro489-Cys630.[Accession | P04626-1] Molecular Weight The protein has a predicted MW of 18.5 kDa. Due to glycosylation, the protein migrates to 28-40 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human Her2 Domain 4, His Tag at 1g/ml (100l/well) on the plate. Dose response curve for Anti-Her2 Antibody, hFc Tag with the EC50 of 13.1ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.