Recombinant Biotinylated Human SIRP alpha V8 Protein,His-Avi Tag

Description :
Synonyms MFR; BIT;CD172a; MYD-1 Source Biotinylated Human SIRP alpha V8 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Glu31-Arg369(S44L, S50T, I52T, H54R, V57A).[Accession | ATD50864.1] Molecular Weight The protein has a predicted MW of 40.1 kDa. Due to glycosylation, the protein migrates to 52-68 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE Activity ELISA Data: Immobilized Human CD47, hFc Tag at 1g/ml (100l/well) on the plate. Dose response curve for Biotinylated Human SIRP alpha V8, His Tag with the EC50 of 0.53ug/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.