Recombinant Human LAG3/CD223 Protein,hFc Tag

Description :
Synonyms LAG3; FDC;CD223; sLAG-3 Source Human LAG3/CD223 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Leu23-Leu450.[Accession | P18627-1] Molecular Weight The protein has a predicted MW of 72.4 kDa. Due to glycosylation, the protein migrates to 75-80 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE Activity ELISA Data: Immobilized Human FGL1, His Tag at 1g/ml (100l/well) on the plate. Dose response curve for Human LAG3, hFc Tag with the EC50 of 47.9ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.