Recombinant Biotinylated Human CTLA-4/CD152 Protein,His-Avi Tag

Description :
Synonyms ICOS; CD152;CTLA4; GRD4 Source Biotinylated Human CTLA-4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Lys36-Asp161.[Accession | P16410] Molecular Weight The protein has a predicted MW of 16.3 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Anti-CTLA-4 Antibody, hFc Tag at 1g/ml (100l/well) on the plate. Dose response curve for Biotinylated Human CTLA-4, His Tag with the EC50 of 45.7ng/ml determined by ELISA . Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.