Recombinant Cynomolgus TPBG/5T4 Protein,His Tag

Description :
Synonyms WAIF1; 5T4AG;M6P1; 5T4 Source Cynomolgus TPBG/5T4 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Thr35-Ser355.[Accession | Q4R8Y9] Molecular Weight The protein has a predicted MW of 35.95 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Cynomolgus TPBG, His Tag at 0.5g/ml (100l/Well) on the plate. Dose response curve for Anti-TPBG Antibody, hFc Tag with the EC50 of 7.1ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.