Recombinant Human JAM-A Protein,His Tag

Description :
Synonyms CD321; JAM-1;JAM-A; JCAM Source Human JAM-A Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ser28-Val238.[Accession | Q9Y624-1] Molecular Weight The protein has a predicted MW of 24 kDa. Due to glycosylation, the protein migrates to 28-35 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE Activity ELISA Data: Immobilized Human JAM-A, His Tag at 0.5g/ml (100l/Well) on the plate. Dose response curve for Anti-JAM-A Antibody, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.