Recombinant Human Siglec-15/CD33L3 Protein,His-Avi Tag

Description :
Synonyms HsT1361; CD33L3;CD33 molecule-like 3; CD33 antigen-like 3 Source Human Siglec-15/CD33L3 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Phe20-Thr263.[Accession | Q6ZMC9] Molecular Weight The protein has a predicted MW of 29.5 kDa. Due to glycosylation, the protein migrates to 30-38 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human Siglec-15, His Tag at 0.5g/ml (100l/well) on the plate. Dose response curve for Anti-Siglec-15 Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in 20mM NaAC (pH 5.0). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in 20mM NaAC (pH 5.0).