Recombinant Biotinylated Human SIRP alpha V2/CD172a Protein,His-Avi Tag

Description :
Synonyms MFR; BIT;CD172a; MYD-1 Source Biotinylated Human SIRP alpha V2/CD172a Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Glu31-Arg369.[Accession | AAH26692.1] Molecular Weight The protein has a predicted MW of 40 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human CD47, hFc Tag at 1g/ml (100l/well) on the plate. Dose response curve for Biotinylated Human SIRP alpha V2, His Tag with the EC50 of 0.36g/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.