Recombinant Biotinylated Human VEGF-C/Flt4-L Protein,His-Avi Tag

Description :
Synonyms Flt4-L; VEGFc;VEGFC; VEGF-C Source Biotinylated Human VEGF-C/Flt4-L Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Thr103-Arg227.[Accession | Q6FH59] Molecular Weight The protein has a predicted MW of 17.1 kDa. Due to glycosylation, the protein migrates to 23-30 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE Activity ELISA Data: Immobilized Biotinylated Human VEGF-C, His Tag at 0.5g/ml (100l/well) on the streptavidin precoated plate (5g/ml). Dose response curve for Human VEGF R3, hFc Tag with the EC50 of 8.4ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in 50mM MES, 150mM NaCl (pH 6.0). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in 50mM MES, 150mM NaCl (pH 6.0).