Recombinant Biotinylated Human Siglec-4a/MAG Protein,His-Avi Tag

Description :
Synonyms Siglec-4a; Siglec4a;MAG; GMA Source Biotinylated Human Siglec-4a/MAG Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gly20-Pro516.[Accession | P20916] Molecular Weight The protein has a predicted MW of 57.6 kDa. Due to glycosylation, the protein migrates to 78-85 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Anti-Siglec-4a Antibody, hFc Tag at 1g/ml (100l/well) on the plate. Dose response curve for Biotinylated Human Siglec-4a, His Tag with the EC50 of 24.4ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.