Recombinant Human TNFRSF11A/Rank Protein,hFc Tag

Description :
Synonyms NF-KB; Receptor activator of NF-KB;ODFR; CD265 Source Human TNFRSF11A/Rank Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Ile30-Pro212.[Accession | Q9Y6Q6-1] Molecular Weight The protein has a predicted MW of 46.85 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human RANKL, His Tag at 2g/ml (100l/well) on the plate. Dose response curve for Human TNFRSF11A, hFc Tag with the EC50 of 8.5ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.