Recombinant Human GFRAL/GFR alpha-like Protein,His-Avi Tag

Description :
Synonyms GRAL; GFRAL;GFR alpha-like; Source Human GFRAL/GFR alpha-like Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ser19-Glu351.[Accession | Q6UXV0] Molecular Weight The protein has a predicted MW of 40.7 kDa. Due to glycosylation, the protein migrates to 48-62 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human GFRAL, His Tag at 0.5g/ml (100l/well) on the plate. Dose response curve for Human GDF15, hFc Tag with the EC50 of 2.5ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4).Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.