Recombinant Human GUCY2C/Guanylyl cyclase C Protein,hFc Tag

Description :
Synonyms STAR; GC-C;Guanylyl cyclase C; GUC2C Source Human GUCY2C/Guanylyl cyclase C Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Ser24-Gln430.[Accession | P25092-1] Molecular Weight The protein has a predicted MW of 72.8 kDa. Due to glycosylation, the protein migrates to 85-115 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human GUCY2C, hFc Tag at 1g/ml (100l/well) on the plate. Dose response curve for Biotinylated Anti-GUCY2C Antibody, hFc Tag with the EC50 of 25.6ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.