Recombinant Cynomolgus 4-1BB Ligand/TNFSF9 Protein,hFc Tag

Description :
Synonyms 4-1BBL; 4-1BB Ligand;41BB Ligand; TNFSF9 Source Cynomolgus 4-1BB Ligand/TNFSF9 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Arg68-Glu251.[Accession | XP_015296398.1] Molecular Weight The protein has a predicted MW of 46 kDa. Due to glycosylation, the protein migrates to 50-52 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Cynomolgus/Rhesus macaque 4-1BB, His Tag at 0.2g/ml (100l/Well) on the plate. Dose response curve for Cynomolgus 4-1BB Ligand, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.