Recombinant Human BAFF/TNFSF13B/CD257 Protein,monomeric hFc Tag

Description :
Synonyms CD257; BLyS;BAFF; TNFSF20 Source Human BAFF/TNFSF13B/CD257 Protein is expressed from HEK293 with hFc tag at the N-Terminus. It contains Ala134-Leu285.[Accession | Q9Y275-1] Molecular Weight The protein has a predicted MW of 41.7 kDa. Due to glycosylation, the protein migrates to 45-48 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 90% as determined by SDS-PAGE and HPLC Activity ELISA Data: Immobilized Human BCMA Trimer, His Tag at 0.5g/ml (100l/Well) on the plate. Dose response curve for Human BAFF, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA . Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.