Recombinant Biotinylated Mouse IL-2 Protein,His-Avi Tag

Description :
Synonyms TCGF; T-cell growth factor;IL2; Source Biotinylated Mouse IL-2 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus. It contains Ala21-Gln169.[Accession | P04351-1] Molecular Weight The protein has a predicted MW of 20.1 kDa. Due to glycosylation, the protein migrates to 27-30 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Mouse IL-2 R alpha, His Tag at 2g/ml (100l/well) on the plate. Dose response curve for Biotinylated Mouse IL-2, His Tag with the EC50 of 65.2ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.