Recombinant Human GFR alpha 1 Protein,His Tag

Description :
Synonyms GDNFR; GFRA1;GDNFRA; RET1L Source Human GFR alpha 1 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Asp25-Ser424.[Accession | NP_665736.1] Molecular Weight The protein has a predicted MW of 45.8 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human GDNF, No Tag at 0.5g/ml (100l/well) on the plate. Dose response curve for Human GFR alpha 1, His Tag with the EC50 of 5.0ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.