Recombinant Human TNFR1/CD120a/TNFRSF1A Protein,hFc Tag

Description :
Synonyms TNFR-I; TNF-RI;TNF-R1; CD120a Source Human TNFR1/CD120a/TNFRSF1A Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Leu30-Thr211.[Accession | P19438-1] Molecular Weight The protein has a predicted MW of 48 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human TNF alpha, His Tag at 0.5g/ml (100l/Well) on the plate. Dose response curve for Human TNFR1, hFc Tag with the EC50 of 8.7ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.