Recombinant Biotinylated Human LAP (TGF beta 1) Protein,His-Avi Tag

Description :
Synonyms TGF beta1; DPD1;CEDLAP; TGFB1 Source Biotinylated Human LAP (TGF beta 1) Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus. It contains Leu30-Arg278(C33S).[Accession | P01137] Molecular Weight The protein has a predicted MW of 31.4 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE Activity ELISA Data: Immobilized Human ITGAV&ITGB6, His Tag at 1g/ml (100l/well) on the plate. Dose response curve for Biotinylated Human LAP (TGF beta 1) , His Tag with the EC50 of 0.86g/ml determined by ELISA .Immobilized Biotinylated Human LAP (TGF beta 1) , His Tag at 0.5g/ml (100l/Well) on the plate. Dose response curve for Human TGF-beta RII, hFc Tag with the EC50 of 23.8ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.