Recombinant Cynomolgus IL-3 R alpha/CD123 Protein,His Tag

Description :
Synonyms CD123; IL3RA; IL3R; IL3RAY Source Cynomolgus IL-3 R alpha/CD123 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Arg18-Arg302.[Accession | G8F3K3-1] Molecular Weight The protein has a predicted MW of 33.9 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity SPR Data: Human IL-3, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus IL-3 R alpha, His Tag with an affinity constant of 0.46 M as determined in SPR assay (QC Test).Cynomolgus IL-3 R alpha, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-3, His Tag with an affinity constant of 1.30 nM as determined in SPR assay . Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.