Recombinant Human TNFSF12 Protein,hFc Tag

Description :
Synonyms DR3LG; APO3L;APO3 ligand; TWEAK Source Human TNFSF12/TWEAK Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Ser43-His249.[Accession | O43508-1] Molecular Weight The protein has a predicted MW of 49.62 kDa. Due to enzyme lysis and glycosylation, the protein migrates to 50-60 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human TNFSF12, hFc Tag at 5g/ml (100l/well) on the plate. Dose response curve for Biotinylated Anti-TNFSF12 Antibody, hFc Tag with the EC50 of 0.11g/ml determined by ELISA . Formulation Lyophilized from 0.22m filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.