Recombinant Mouse IL-7R alpha/CD127 Protein,hFc Tag

Description :
Synonyms CD127; IL7Ra;IL-17R alpha; ILRA Source Mouse IL-7R alpha/CD127 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Glu21-Asp239.[Accession | NP_032398.3] Molecular Weight The protein has a predicted MW of 51.74 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human IL-7, No Tag at 2g/ml (100l/well) on the plate. Dose response curve for Mouse IL-7R alpha, hFc Tag with the EC50 of 10.2ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.