Recombinant Human B7-H4 Protein,His-Avi Tag

Description :
Synonyms B7S1; B7-H4;B7H4; B7x Source Human B7-H4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Phe29-Ala258.[Accession | Q7Z7D3-1] Molecular Weight The protein has a predicted MW of 28.2 kDa. Due to glycosylation, the protein migrates to 52-68 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human B7-H4, His Tag at 0.5g/ml (100l/Well) on the plate. Dose response curve for Anti-B7-H4 Antibody, hFc Tag with the EC50 of 9.4ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.