Recombinant Human Integrin alpha M beta 2 (ITGAM&ITGB2) Heterodimer Protein,His Tag

Description :
Synonyms ITGAM; ITGAM&ITGB2;M2; Source Human Integrin alpha M beta 2 (ITGAM&ITGB2) Heterodimer Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Phe17-Asn1104(ITGAM) acidic tail&Gln23-Asn700(ITGB2) basic tail.[Accession | P11215-1(ITGAM)&P05107-1(ITGB2)] Molecular Weight The protein has a predicted MW of 126.10 kDa(ITGAM)&79.50 kDa(ITGB2). Due to glycosylation, the protein migrates to 140-160 kDa (ITGAM)&90-100 kDa (ITGB2) based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human ITGAM&ITGB2, His Tag at 1g/ml (100l/well) on the plate. Dose response curve for Anti-ITGB2 Antibody, hFc Tag with the EC50 of 13.7ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.