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Product Name :
Recombinant Biotinylated Human Siglec-10 (R119A) Protein,His-Avi Tag

Description :
Synonyms MGC126774; SIGLEC10;SLG2; Source Biotinylated Human Siglec-10(R119A) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Met17-Thr546.[Accession | Q96LC7-1] Molecular Weight The protein has a predicted MW of 62.64 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Biotinylated Human Siglec-10 (R119A) , His Tag at 2g/ml (100l/Well) on the streptavidin (5g/ml) precoated plate. Dose response curve for Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in 20mM Tirs 150mM NaCl, 200mM L-arginine (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage :
The product should be stored at -25~-15℃ for 1 year from date of receipt.

MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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