Recombinant Biotinylated Human TNF alpha Protein,His-Avi Tag

Description :
Synonyms DIF; Cachectin;APC1 protein; TNFalpha Source Biotinylated Human TNF alpha Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Val77-Leu233.[Accession | P01375] Molecular Weight The protein has a predicted MW of 20.3 kDa. Due to glycosylation, the protein migrates to 21-24 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human TNF R2, mFc Tag at 0.5g/ml (100l/Well) on the plate. Dose response curve for Biotinylated Human TNF alpha, His Tag with the EC50 of 5.1ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.