Product Name :
Recombinant Biotinylated Mouse GFRAL/GFR alpha-like Protein,His-Avi Tag
Description :
Synonyms GRAL; GFRAL;GFR alpha-like; Source Biotinylated Mouse GFRAL/GFR alpha-like Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus. It contains Gln20-Glu350.[Accession | Q6SJE0-1] Molecular Weight The protein has a predicted MW of 40.1 kDa. Due to glycosylation, the protein migrates to 48-60 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Biotinylated Mouse GFRAL, His Tag at 0.5g/ml (100l/Well) on streptavidin (5g/ml) precoated plate. Dose response curve for Mouse GDF15, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage :
The product should be stored at -25~-15℃ for 1 year from date of receipt.
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