Product Name :
Recombinant Human PD-1/PDCD1 Protein,hFc Tag
Description :
Synonyms CD279; PD1;PDCD1; PD-1 Source Human PD-1/PDCD1 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Leu25-Gln167.[Accession | Q15116-1] Molecular Weight The protein has a predicted MW of 42.6 kDa. Due to glycosylation, the protein migrates to 65-72 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human PD-1, hFc Tag at 0.5g/ml (100l/Well). Dose response curve for Biotinylated Anti-PD1 Antibody, hFc Tag with the EC50 of 14.6ng/ml determined by ELISA .Immobilized Human PD-1, hFc Tag at 1g/ml (100ul/Well). Dose response curve for Human PD-L1, mFc Tag with the EC50 of 0.1g/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage :
The product should be stored at -25~-15℃ for 1 year from date of receipt.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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