Recombinant Human Alkaline phosphatase Protein (Intestinal type) /ALPI,His-Flag Tag

Description :
Synonyms IAP; APTNAP; ALPI; EC 3.1.3.1 Source Human Alkaline Phosphatase (Intestinal type) /ALPI Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus. It contains Val20-Asp503.[Accession | P09923] Molecular Weight The protein has a predicted MW of 54.5 kDa. Due to glycosylation, the protein migrates to 63-73 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity SPR Data: Anti-Alkaline Phosphatase Antibody captured on CM5 Chip via Protein A can bind Human Alkaline Phosphatase (Intestinal type) , His Tag with an affinity constant of 1.58 M as determined in SPR assay . Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.