Recombinant Biotinylated Mouse PD-1/PDCD1 Protein (Primary Amine Labeling) ,hFc Tag

Description :
Synonyms PDCD1; SLEB2; CD279; Source Biotinylated Mouse PD-1/PDCD1 Protein (Primary Amine Labeling) is expressed from HEK293 with hFc tag at the C-Terminus. It contains Leu25-Gln167.[Accession | NP_032824] Molecular Weight The protein has a predicted MW of 42.9 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity SPR Data: Mouse PD-L1, His Tag captured on CM5 Chip via anti-his antibody can bind Biotinylated Mouse PD-1, hFc Tag with an affinity constant of 3.35 M as determined in SPR assay . Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.