Recombinant Human Notch 1 Protein,His-Avi Tag

Description :
Synonyms Notch-1; 9930111A19Rik; NOTCH1; Mis6 Source Human Notch 1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ala19-Gln526.[Accession | P46531] Molecular Weight The protein has a predicted MW of 56.5 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity SPR Data: Human DLL4, hFc Tag captured on CM5 Chip via Protein A can bind Human Notch 1, His Tag with an affinity constant of 0.48 M as determined in SPR assay . Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.