Recombinant Human PRNP Protein,hFc Tag

Description :
Synonyms ALTPRP;PrP;Major prion protein;PRP Source Human PRNP Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Lys23-Ser230.[Accession | P04156] Molecular Weight The protein has a predicted MW of 49.4 kDa. Due to glycosylation, the protein migrates to 60-67 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE Activity ELISA Data: Immobilized Human PRNP, hFc Tag at 2g/ml (100l/well) on the plate. Dose response curve for Biotinylated Anti-PRNP Antibody, hFc Tag with the EC50 of 33.2ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.