Recombinant Human 2B4/CD244/SLAMF4 Protein,hFc Tag

Description :
Synonyms CD244; CD244 antigen;2B4; NAIL Source Human 2B4/CD244/SLAMF4 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Cys22-Arg221.[Accession | Q9BZW8-2] Molecular Weight The protein has a predicted MW of 49 kDa. Due to glycosylation, the protein migrates to 68-75 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human CD48, His Tag at 1g/ml (100l/well) on the plate. Dose response curve for Human 2B4, hFc Tag with the EC50 of 0.45g/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.