Recombinant Human Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein,His-Avi Tag

Description :
Synonyms beta3;ITGAV&ITGB3;Integrin alpha V beta 3;V3 Source Human Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Phe31-Val992(ITGAV) acidic tail&GLy27-Asp718(ITGB3) basic tail.[Accession | P06756-1(ITGAV)&P05106-1(ITGB3)] Molecular Weight The protein has a predicted MW of 114 kDa (ITGAV)&81.3 kDa (ITGB3). Due to glycosylation,the protein migrates to 85-140 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human ITGAV&ITGB3, His Tag at 2g/ml (100l/well) on the plate. Dose response curve for Anti-alpha V beta3 Antibody, hFc Tag with the EC50 of 9.2ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.