Recombinant Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer Protein,His-Avi Tag

Description :
Synonyms CT6.1;CTAG1;MY-ESO-1;MHC Source Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer Protein is expressed from E.coli with His tag and Avi tag at the C-Terminus,tetramer is assembled by biotinylated monomer and streptavidin. It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and SLLMWITQC peptide.[Accession | A0A140T913(HLA-A*02:01)&P61769(B2M)&SLLMWITQC] Molecular Weight The protein has a predicted MW of 258 kDa. The protein migrates to 160-250 kDa (HLA-A*02:01&streptavidin tetramer) and 11.9 kDa (B2M) based on SDS-PAGE result under Non reducing (N) condition. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer, His Tag at 5g/ml (100l/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Antibody, hFc Tag with the EC50 of 1.9ng/ml determined by ELISA. Formulation Lyophilized from 0.22 m filtered solution in 20mM Tris, 150mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.