Recombinant Human tPA Protein,His Tag

Description :
Synonyms t-PA; tPA; Alteplase; Reteplase; PLAT; Source Human tPA Protein is expressed from HEK293 with His tag at the C-terminus. It contains Ser36-Pro562, which consists of two chains: chain A (Ser36-Arg310) and chain B (Ile311-Pro562).[Accession | P00750-1] Molecular Weight The protein has a predicted MW of 60.13 kDa. The protein is activated by thermolysin and cleaved to a 2-chain protease, it migrates to 40-45 kDa (Chain A) and 35-40 kDa (Chain B) based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE Formulation Lyophilized from 0.22m filtered solution in 20mM MES, 300mM Nacl, 200mM Arginine (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in 20mM MES, 300mM Nacl, 200mM Arginine (pH 5.5).