Recombinant Human Integrin alpha V beta 1 (ITGAV&ITGB1) Heterodimer Protein,His-Avi Tag

Description :
Synonyms Integrin alpha V beta 1;V1;ITGAV&ITGB1 Source Human Integrin alpha V beta 1 (ITGAV&ITGB1) Heterodimer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Phe31-Val992 (ITGAV) acidic tail & Gln21-Asp728 (ITGB1) basic tail.[Accession | P06756-1(ITGAV)&P05556-1(ITGB1)] Molecular Weight The protein has a predicted MW of 114 kDa (ITGAV) and 83.2 kDa (ITGB1). Due to glycosylation, the protein migrates to 130-160 kDa (ITGAV) and 100-120 kDa (ITGB1) based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human ITGAV&ITGB1, His Tag at 2g/ml (100l/well) on the plate. Dose response curve for Anti-alpha V Antibody, hFc Tag with the EC50 of 14.4ng/ml determined by ELISA. Formulation Lyophilized from 0.22 m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.