Recombinant Human CD8 alpha&beta Heterodimer Protein,His-Flag Tag

Description :
Synonyms CD8A&CD8B;CD8 alpha&beta Source Human CD8 alpha&beta Heterodimer Protein is expressed from HEK293 with His tag and Flag tag at the C-Terminus. It contains Ser22-Asp182(CD8 alpha) acidic tail and Leu22-Pro170(CD8 beta) basic tail.[Accession | P01732-1(CD8 alpha)&P10966-1(CD8 beta)] Molecular Weight The protein has a predicted MW of 23.54 kDa (CD8 alpha) and 22.62 kDa (CD8 beta). Due to glycosylation, the protein migrates to 26-28 kDa (CD8 alpha) and 28-32 kDa (CD8 beta) based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human CD8A&CD8B, His Tag at 0.5g/ml (100l/well) on the plate. Dose response curve for Anti-CD8 Antibody, hFc Tag with the EC50 of 15.2ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.