Recombinant Human Neogenin Protein,hFc Tag

Description :
Synonyms NGN;neogenin 1;IGDCC2;NEO1 Source Human Neogenin Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Ala34-Leu1105.[Accession | Q92859-1] Molecular Weight The protein has a predicted MW of 144.37 kDa. Due to glycosylation, the protein migrates to 150-180 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human RGMa, His Tag at 5g/ml (100l/Well) on the plate. Dose response curve for Human Neogenin, hFc Tag with the EC50 of 0.17g/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.