Recombinant Human IL-23 alpha&IL-12 beta Protein,His Tag

Description :
Synonyms IL23 alpha&IL12 beta ;IL23 alpha&IL12 ;IL12 beta;IL23 alpha Source Human IL-23 alpha&IL-12 beta Protein is expressed from HEK293 with His tag at the C-terminus. It contains Arg20-Pro189(IL-23 alpha) & Ile23-Ser328(IL-12 beta).[Accession | Q9NPF7(IL-23 alpha)&P29460(IL-12 beta)] Molecular Weight The protein has a predicted MW of 19.77 kDa (IL-23 alpha) and 34.7 kDa (IL-12 beta). Due to glycosylation, the protein migrates to 23 kDa (IL-23 alpha) and 40-50 kDa (IL-12 beta) based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human IL-23 alpha&IL-12 beta, His Tag at 1g/ml (100l/well) on the plate. Dose response curve for Anti-IL-23 Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA.ELISA Data: Immobilized Human IL-23R, hFc Tag at 2g/ml (100l/well) on the plate. Dose response curve for Human IL-23 alpha&IL-12 beta, His Tag with the EC50 of 0.12g/ml determined by ELISA. Formulation Lyophilized from 0.22 m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.