Recombinant Cynomolgus/Rhesus macaque B7-H5/Gi24/VISTA Protein,His Tag

Description :
Synonyms B7H5; B7-H5; VISTA; VSIR ; PD1H; PD-1H; 4632428N05Rik; C10orf54; Dies1; Gi24; PP2135; SISP1 Source Cynomolgus/Rhesus macaque B7-H5/Gi24/VISTA Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Phe33-Thr192.[Accession | XP_005565644.2 / F7F6S6] Molecular Weight The protein has a predicted MW of 19.13 kDa. Due to glycosylation, the protein migrates to 40-60 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Cynomolgus/Rhesus macaque B7-H5, His Tag at 0.5g/ml (100l/Well) on the plate. Dose response curve for Anti-B7-H5 Antibody, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA. Formulation Lyophilized from 0.22 m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.