Recombinant Cynomolgus CD3E&CD3D/CD3 epsilon&CD3 delta Protein,His-Flag Tag

Description :
Synonyms CD3; CD3e; CD3E; CD3d; T3D; CD3d; CD3E&CD3d; CD3 delta&CD3 epsilon Source Cynomolgus CD3E&CD3d/CD3 epsilon&CD3 delta Protein is expressed from HEK293 with His tag and Flag tag at the C-Terminus. It contains Gln22-Asp117(CD3E) & Phe22-Ala105(CD3d).[Accession | Q95LI5.2(CD3E)&Q95LI8(CD3d)] Molecular Weight The protein has a predicted MW of 11.93 kDa (CD3E) and 10.55 kDa (CD3d). Due to glycosylation, the protein migrates to 13-20 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Cynomolgus CD3E&CD3d, His Tag at 0.5g/ml (100l/well) on the plate. Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA. Formulation Lyophilized from 0.22 m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.