Recombinant Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein,hFc-Avi Tag

Description :
Source Recombinant Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein is expressed from HEK293 with hFc tag and Avi tag at the C-terminal. It contains Asp23-Asp126(CD3E) & Phe22-Ala105(CD3D).[Uniprot|P07766(CD3E)&P04234(CD3D)] Molecular Weight The protein has a predicted MW of 40 kDa (CD3E) and 35.4 kDa (CD3D). Due to glycosylation,the protein migrates to 48-60 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGEandHPLC Activity ELISA Data: Immobilized Biotinylated Human CD3E&CD3D, hFc Tag at 1g/ml (100 l/Well) on streptavidin (5g/ml) precoated plate. Dose response curve for OKT3, mFc Tag with the EC50 of 42.1ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.